Operationalizing point-of-need DNA detection systems to assess risk of invasive dreissenid dispersal vectors

Funding period: 2022-2025
Lead: Rob Bajno
Total GRDI funding: $190,570

Acceptance of environmental DNA (eDNA) data in decision support frameworks within resource management, conservation, and enforcement programs, has predictably increased the need for eDNA information. Furthermore, as new eDNA and eRNA (environmental RNA) methodologies emerge, their utility to support expanding organizational needs is being recognised. This project develops in-field or point-of-need DNA detection methods, to an operational level for the detection of invasive mussels from high risk dispersal vectors (aquaria and watercraft) in an effort to expedite management response decisions. This study also tests the utility of eRNA (RNA is only produced by living organisms) to augment eDNA results, thereby enhancing early detection and response work, which is an inherent and key component of AIS programming across Canada. We will evaluate the benefits and limitations of point-of-need eDNA and eRNA detection approaches to support a broader national effort to combat invasive dreissenids. Outputs will build a foundation for transferring methods to other aquatic species.