Funding period: 2020-2024
Lead: Amanda Roe
Total GRDI funding: $179,220
This project aims to develop diagnostic tools for genomic monitoring to track second diapause across Spruce Budworm (SBW) populations to enhance monitoring and suppression efforts. Diapause is a critical life stage that allows SBW to survive harsh winter conditions. A second diapause stage can occur and its prevalence varies among populations and environmental conditions, creating mixed populations of one-year and two-year lifecycles. Second diapause is genetically controlled and we will identify the genes or regions responsible for this trait. Characterizing this trait will increase our understanding of SBW biology and inform spruce budworm management and suppression efforts. These data will form the foundation for a genomic monitoring tool to track second diapause across populations.
Publications
- Beliveau C, Gagne P, Picq S, Vernygora O, Keeling CI, Pinkney K, Doucet D, Wen F, Johnston JS, Maaroufi H, Boyle B, Laroche J, Dewar K, Juretic N, Blackburn G, Nisole A, Brunet B, Brandao M, Lumley L, Duan J, Quan G, Lucarotti CJ, Roe AD, Sperling FAH, Levesque RC, Cusson M. 2022. The Spruce Budworm Genome: Reconstructing the Evolutionary History of Antifreeze Proteins. Genome Biology and Evolution https://doi.org/10.1093/gbe/evac087
- Butterson S, Roe AD, Marshall KE. 2021. Plasticity of cold hardiness in the eastern spruce budworm, Choristoneura fumiferana. Comp Biochem Physiol A Mol Integr Physiol, 110998. https://doi.org/10.1016/j.cbpa.2021.110998
- Marshall KE, Roe AD. 2021. Surviving the frozen forest: the physiology of spruce budworm overwintering. American Journal of Physiology. 36(3): 174-182. https://doi.org/10.1152/physiol.00037.2020
- Perrault K, Wardlaw AA, Candau JN, Irwin CL, Demidovich M, MacQuarrie CJK, Roe AD. 2021. From branch to bench: establishing wild spruce budworm populations into laboratory colonies for the exploration of local adaptation and plasticity. Can Entomol, 1-17. https://doi.org/10.4039/tce.2021.1
- Roe AD, Wardlaw AA, Butterson S, Marshall KE. 2024. Diapause survival requires a temperature sensitive preparatory period. Curr Res Insect Sci. e100073. https://doi.org/10.1016/j.cris.2024.100073
- Wardlaw AA, Perrault K, Roe AD, Dedes J, Irwin CL, MacQuarrie CJK, Candau JN. 2021. Methods for estimating and modelling spruce budworm development rates at constant temperatures. Can Entomol 154 https://doi.org/10.4039/tce.2021.56
Contact us
For additional information, please contact:
Genomics R&D Initiative
Email: info@grdi-irdg.collaboration.gc.ca